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Polychlorinated biphenyls PCBs Essay: Due to their high lipophilicity and chemical stability, they are widely existed, and may biomagnify through the food chain and bioaccumulate in organisms. PCBs cause a variety of toxic effects, affecting immune, endocrine, nervous and reproductive system Arnold et al.
The majority of toxic effects elicited by coplanar PCBs are mediated by its binding to the aryl hydrocardon receptor AhRand subsequent induction of responsive genes Barouki et al.
In addition, one of the important mechanisms of PCBs toxicity is inflammation Hennig et al. Importantly, the thyroid axis appears to be susceptible to the influence of PCBs Murk et al.
However, it is not known whether inflammation would play a role in these adverse effects of PCBs. A few studies indicated that environmental pollutants, especially PCBs, are potential contributors to the development of autoimmune thyroiditis Langer et al.
Pro-inflammatory cytokines are involved in the pathogenesis of autoimmune and proliferative thyroid disorders Ajjan et al. They are produced by immune cells as well as thryocytes and lymphocytes Ajjan et al. Moreover, inflammatory cytokines could cause local vasodilatation of the thyroid, increasing permeability of vascular endothelium and tissue liquid exudation, resulting in thyroid dysfunction and structure disruption George and Chrousos Methods and materials Materials.
Newborn calf serum was obtained from Gibco USA. Cell culture and treatment. Hedwig Hospital Lan et al. FRTL-5 cells were cultured as we previously described Yang et al.
Cells were treated with indicated concentrations of PCB 0. The selection of the low concentrations of PCB was based on our early viability and apoptosis assays, ranging from 0. FRTL-5 cells were plated in six-well plates 2ml, 1.
Cells were then treated with PCB at concentrations of 0. Sequences of the primers we used are listed in Table 1. All primers were synthesized by Takara. FRTL-5 cells were cultured in six-well plates 2 mL, 1.
Equal amounts of protein 30?? Protein visualization was performed using an enhanced chemiluminescence reagent Thermo, USA. Cell lysates were extracted 24 h or 48 h later to isolate RNA or protein, separately.
Aryl hydrocarbon receptor AhR inhibitor treatment. Animal treatment and sample collection. After one week acclimation, rats were randomly divided into four groups, and administered vehicle corn oil, 0.
Body weights were measured daily. At the study end point, rats were euthanized with an overdose of i.
All animals received humane care and studies have been approved by the Institutional Animal Care and Use Committee. Sections were deparaffinized and stained with hematoxylin and eosin HE and observed under light microscope.
C for 1 hour, deparaffinized with xylenes, and rehydrated using graded ethanol and distilled water. Antigen retrieval was conducted using citrate buffer. C, followed by horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG Bioworld. Results Effects of PCB on body weight and cell viability No clinical signs of toxicity or morbidity were observed among animals following exposure to PCBMay 20, · A polychlorinated biphenyl (PCB) is an organic chlorine compound with the formula C H skybox2008.comlorinated biphenyls were once widely deployed as dielectric and coolant fluids in electrical apparatus, carbonless copy paper and in heat transfer fluids.
 Because of their longevity, PCBs are still widely in use, even though their manufacture has declined drastically since the s, when a host. Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs), which are worldwide problem.
Analysis of PCBs in oil is generally di fficult because there are congeners and similar chemi cal substances in oil. Note: Citations are based on reference standards.
However, formatting rules can vary widely between applications and fields of interest or study. The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied. Food and Beverage Analysis Analysis of Polychlorinated Biphenyls in Fish Oil Using Supelclean EZ-POP NP, Silica Gel SPE, and an SLB-5ms GC Column Kathy Stenerson1, Principle R&D Scientist; Caitlin Brown2, Summer Intern Analysis of Polychlorinated Biphenyls in Fish Oil.
METHOD A POLYCHLORINATED BIPHENYLS (PCBs) BY GAS CHROMATOGRAPHY SW is not intended to be an analytical training manual.
Therefore, method procedures are written based on the assumption that they will be followed by individuals formally trained in at least the basic principles of chemical .
PCBs (polychlorinated biphenyls) have been used industrially since Industrial mixtures of PCBs are known by commercial names, e.g., Aroclors (United States), Kaneclor (Japan), Chlophen (Germany), Sovol (former USSR), Fenchlor (France).